{"id":3027520,"url":"https://alion.io/job/alnylam-principal-scientist","title":"Principal Scientist, Biotransformation and Metabolite Profiling","company":{"id":1776651,"name":"Alnylam","domain":"alnylam.com","url":"https://alion.io/company/alnylam","size_band":"51-200","is_staffing_agency":false,"employer_type":"direct","is_intermediary":false,"listed_via":null,"ats_vendor":"Greenhouse","truth_index":{"grade":"A","score":100,"open_postings":3,"ghost_share":0,"stale_share":0,"repost_share":0,"time_to_fill_p50_days":27,"computed_at":"2026-11-01T06:10:25Z"}},"role":"Science & Research","role_family":"Science & Research","seniority":"lead","employment_type":null,"work_mode":"on_site","remote_scope":null,"remote_scope_basis":null,"remote_working_hours":null,"hiring_geo_confidence":"structured","locations":["Cambridge, United States","United States"],"countries":["US"],"hiring_countries":[],"hiring_countries_total":0,"salary":null,"salary_estimate":{"min_usd":87000,"max_usd":241000,"period":"year","method":"role_seniority_country_remote_cell","sample_n":541},"experience_years_min":6,"visa_sponsorship":false,"relocation_package":false,"has_equity":false,"technologies":[{"name":"Chromatography","optional":false},{"name":"GLP","optional":false},{"name":"Mass Spectrometry","optional":false}],"status":"live","first_seen_at":"2026-10-21T15:32:07Z","employer_posted_date":"2026-10-22","last_verified_at":"2026-11-01T11:45:51Z","board_verified":true,"closed_at":null,"days_open":10,"trust":{"level":"ok","repost_count":null,"flags":[],"days_open":10},"description":"Overview:\nWe are seeking an experienced and highly motivated Principal Scientist, Biotransformation & Metabolite Profiling to provide scientific leadership in the characterization of drug metabolism and biotransformation across discovery and development programs.\nThe successful candidate will develop and execute integrated metabolite profiling and identification strategies using state-of-the-art LC-HRMS/MS approaches. The role will support a range of therapeutic modalities, including small molecules, peptides, RNAi, conjugated therapeutics, and other emerging modalities.\nThe scientist will serve as a subject-matter expert in metabolite identification and biotransformation, partnering closely with DMPK, bioanalytical sciences, pharmacology, toxicology, research/biology, and other project functions to translate metabolite data into actionable understanding of drug disposition, efficacy, and safety.\nKey Responsibilities: \nLead metabolite profiling and structural identification in plasma, urine, bile, tissues, and relevant in vitro biological matrices.\nSupport a range of therapeutic modalities, including small molecules, peptides, RNAi, conjugated therapeutics, and other emerging modalities.\nApply LC-HRMS/MS approaches for metabolite detection, profiling, structural elucidation, and relative quantitation.\nExtensively and proficiently conduct advanced MS data processing platforms, specifically Thermo Scientific Compound Discoverer, BioPharma Finder, ProMass, Mass MetaSite and other metabolite profiling software.\nDesign appropriate biotransformation strategies using hepatocytes, liver subcellular fractions, lysosomal systems, serum/plasma, tissue preparations, and other fit-for-purpose models.\nCharacterize metabolic pathways including oxidation, reduction, hydrolysis, conjugation, proteolysis, deconjugation, linker cleavage, and other modality-specific catabolic pathways.\nMay support PK and PD analysis as needed.\nEstablish relationships between in vitro metabolism, in vivo circulating metabolites, and tissue-specific metabolism and catabolism.\nIdentify major, disproportionate, persistent, or pharmacologically relevant metabolites and provide scientific interpretation of their potential significance.\nInterpret accurate mass, isotope patterns, charge states, MS/MS fragmentation, product-ion spectra, and chromatographic behavior to support metabolite assignments.\nEvaluate and implement new mass spectrometry technologies, software, and data-processing approaches that improve metabolite identification efficiency and confidence.\nConduct non-GLP sample analysis to support all Alnylam’s RNAi therapeutic development from DC selection and CTA/IND enabling to late stages.\nResponsible for staff coaching/career development, goal setting, and performance evaluation.\nInteract with research, eDEV, and clinical teams for the responsible programs and build strong working relationships via teamwork. Serve as BA representative for the responsible programs.\nCollaborate with cross-functional teams within Research and Development to align projects and timelines, and aid in interpretation of data.\nAuthor BA reports for in vitro and in vivo metabolite profiling used in IND filings, and support method transfers to CROs, as needed.\nQualifications: \nPhD in analytical chemistry, medicinal chemistry, pharmaceutical sciences, biochemistry, drug metabolism with 6+ years of relevant industry research experience in small molecule, peptide, oligonucleotide, RNAi development.\nExtensive hands-on experience in drug metabolism, metabolite profiling, and metabolite identification for small molecules, peptide, oligonucleotide, ADC, or RNAi compounds.\nStrong expertise in LC-MS/MS and high-resolution mass spectrometry, including interpretation of complex MS/MS fragmentation data.\nDemonstrated ability to elucidate metabolite structures and construct scientifically supported biotransformation pathways.\nStrong understanding of drug metabolism, PK, tissue distribution, and mechanisms governing metabolite formation.\nTrack record in preparing conference presentations, publications in peer reviewed journals, or industry and regulatory white papers.\nDetail-oriented with exceptional organizational skills, the ability to handle multiple projects, and thrive in a fast-paced, team-oriented environment.\nPossesses excellent verbal and written communication skills.\nAbout Alnylam\nWe are the leader in RNAi therapeutics - a revolutionary approach with the potential to transform the lives of people with rare and common diseases. Built on Nobel Prize-winning science, Alnylam has delivered the breakthroughs that made RNAi therapeutics possible and are just at the beginning of what’s possible. Our deep pipeline, late-stage programs, and bold vision reflect our core values: fierce innovation, passion for excellence, purposeful urgency, open culture and commitment to people. We're proud to be a globally recognized top employer, where an authentic, inclusive culture and breakthrough thinking fuel one another.\nAt Alnylam, we commit to an inclusive recruitment process and equal employment opportunity. Qualified applicants will receive consideration for employment without regard to their sex, gender or gender identity, sexual orientation, race, color, ethnicity, national origin, ancestry, citizenship, religion, creed, physical or mental disability, pregnancy status or related conditions, genetic information, veteran or military status, marital or familial status, political affiliation, age, or any other factor protected by federal, state, or local law. Alnylam is an E-Verify Employer.\nBefore applying, please review Alnylam's Candidate Privacy Notice regarding how Alnylam handles Personal Data you provide with your application for employment.","description_format":"text","description_chars":5774,"description_truncated":false,"requirements":{"experience_years_min":6,"management_years_min":null,"team_size_min":null,"manages_managers":false,"education":{"level":"master","optional":false},"security_clearance":false,"languages":[]},"benefits":[],"hiring_locations":[],"hiring_excludes":[],"relocation_offered":false,"industries":["Cell & Gene Therapy","Rare Disease Therapeutics","Cardiometabolic Therapeutics"],"lifecycle":[{"event":"open","at":"2026-10-21T19:29:29Z"}],"visa":[{"country":"US","licensed_sponsor":true,"evidence":"H-1B filings in 12 months: 36 · green card filings: 9","filings_12m":36,"filings_prev_12m":48,"green_card_filings_12m":9,"median_offered_wage_usd":157500,"route":null,"cap_exempt":false,"checked_at":"2026-10-29T16:27:21+00:00","sources":["US Department of Labor: LCA disclosure data (H-1B, H-1B1, E-3)","US Department of Labor: PERM disclosure data (green cards)"],"filings_for_role_12m":0}],"liveness":{"score":75,"band":"hot","label":"Hiring now","p_open":1,"p_active":0.835,"p_room":0.9,"age_days":10,"expected_fill_days":27,"reasons":["conf:2","win:mid"],"computed_at":"2026-11-01T06:10:25Z"},"pay":null,"html_url":"https://alion.io/job/alnylam-principal-scientist","json_url":"https://alion.io/job/alnylam-principal-scientist.json","meta":{"generated_at":"2026-11-01T14:00:04Z","cache_seconds":300,"methodology":"https://alion.io/methodology","terms":"https://alion.io/terms","contact":"https://alion.io/contact","api":"https://alion.io/developers","about":"Alion is a live layer of people, companies and AI agents: who they are, whether they are real and active right now, what they do and how to work with them, readable by people and by agents and paid per call.","catalog":"https://alion.io/catalog.json","usage":{"tier":"crawler_verified","counted_by":"address","units_charged":1,"used_today":21553,"day_limit":null,"remaining_today":null,"minute_limit":300,"resets_at":"2026-11-02T00:00:00Z"}},"offers":[{"id":"company.slices","title":"One company in depth, by slice","status":"live","price":{"credits":0.02,"usd":0.002,"plus_per_slice":{"credits":0.05,"usd":0.005}},"unit":"per company, plus each slice with data","note":"the employer in depth","call":{"mcp_tool":"get_company","arguments":{"id":1776651},"rest":"https://alion.io/mcp/rest/get_company?id=1776651"},"human":"https://alion.io/catalog?offer=company.slices&for=job%2Falnylam-principal-scientist"},{"id":"market.stats","title":"A market slice: pay, demand and time to fill","status":"live","price":{"credits":1,"usd":0.1},"unit":"per slice","note":"pay, demand and time to fill for this role and place","call":{"mcp_tool":"market_stats"},"human":"https://alion.io/catalog?offer=market.stats&for=job%2Falnylam-principal-scientist"},{"id":"job.search","title":"Open jobs by role, technology, place, pay and visa","status":"live","price":{"credits":0.02,"usd":0.002},"unit":"per posting in a list","note":"similar open postings","call":{"mcp_tool":"search_jobs"},"human":"https://alion.io/catalog?offer=job.search&for=job%2Falnylam-principal-scientist"},{"id":"company.verify","title":"Is this company real and active right now","status":"pilot","price":null,"unit":"per company","request":{"url":"https://alion.io/catalog/request","method":"POST","body":"{\"offer\": \"company.verify\", \"for\": \"job/alnylam-principal-scientist\", \"note\": \"what you need it for\"}"},"human":"https://alion.io/catalog?offer=company.verify&for=job%2Falnylam-principal-scientist"}],"agent":{"mcp":"https://alion.io/mcp/jobs","mcp_open":"https://alion.io/mcp/open","next":[{"tool":"get_job","arguments":{"id":3027520}},{"tool":"search_jobs","arguments":{"company":"alnylam.com","detail":"compact"}},{"tool":"match_people","arguments":{"job_id":3027520},"note":"people open to offers who fit this vacancy"}],"note":"The same data through MCP tools: https://alion.io/mcp/open needs no sign-in (compact searches, postings, people); https://alion.io/mcp with an Alion account has everything. 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