{"id":2634231,"url":"https://alion.io/job/axleinfo-cell-line-development-scientist","title":"Cell Line Development Scientist","company":{"id":4416199,"name":"Copyright Axle Informatics","domain":"axleinfo.com","url":"https://alion.io/company/axleinfo","size_band":null,"is_staffing_agency":false,"employer_type":"direct","is_intermediary":false,"listed_via":null,"ats_vendor":"Greenhouse","truth_index":{"grade":"B","score":75,"open_postings":6,"ghost_share":0,"stale_share":1,"repost_share":0,"time_to_fill_p50_days":null,"computed_at":"2026-10-16T05:45:00Z"}},"role":"Science & Research","role_family":"Science & Research","seniority":null,"employment_type":null,"work_mode":"on_site","remote_scope":null,"remote_scope_basis":null,"remote_working_hours":null,"hiring_geo_confidence":"structured","locations":["Gaithersburg, United States"],"countries":["US"],"hiring_countries":[],"hiring_countries_total":0,"salary":{"min":100000,"max":110000,"currency":"USD","period":"year","gross":null,"usd_annual":110000},"salary_estimate":null,"experience_years_min":null,"visa_sponsorship":false,"relocation_package":false,"has_equity":false,"technologies":[{"name":"Microsoft Excel","optional":false}],"status":"live","first_seen_at":"2026-10-14T15:50:22Z","employer_posted_date":"2026-10-14","last_verified_at":"2026-10-16T21:26:43Z","board_verified":true,"closed_at":null,"days_open":2,"trust":{"level":"ok","repost_count":null,"flags":[],"days_open":2},"description":"(ID: 2026-3798)\nAxle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).\nAxle is seeking a Cell Line Development Scientist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute of Allergy and Infectious Diseases (NIAID) located in Gaithersburg, MD.\nBenefits We Offer:\n100% Medical, Dental & Vision Coverage for Employees\nPaid Time Off and Paid Holidays\n401K match up to 5%\nEducational Benefits for Career Growth\nEmployee Referral Bonus\nFlexible Spending Accounts:Healthcare (FSA)\nParking Reimbursement Account (PRK)\nDependent Care Assistant Program (DCAP)\nTransportation Reimbursement Account (TRN)\n\nPosition Overview\nThe Cell Line Development Scientist will support the planning and execution of research experiments within the laboratory’s overall research program. The role focuses on developing stable cell lines for vaccine and therapeutic protein candidates, including monoclonal antibodies, recombinant proteins, and virus-like particles. It covers the full cell line development process, from plasmid preparation and transfection through clone selection, fed-batch analysis, and stability studies. The role also includes small-scale process development, laboratory equipment support, documentation, and project coordination with project leads. A Master’s degree is required.\nCertifications & Licenses\nMaster of Science (M.S.) in Biological Science\n\nBachelor of Science (B.S.) in Biological Sciences\n\nField of Study\nBiochemical Sciences\n\nSoftware\nPrism, FlowJo, Word, PowerPoint, Excel\n\nSkills\nMonoclonal antibody and/or vaccine production\n\nTechnical reports, standard operating procedures, and data presentations\n\nCell line engineering technologies, including cell counting, high-throughput transfection, single-cell cloning, and flow cytometry\n\nAseptic technique and mammalian cell culture\n\nBroad knowledge of state-of-the-art principles and theory\n\nDeliverables\nProvide reports (Ad-Hoc)\n\nStatement of Work Details\nContribute to the development of stable cell lines for vaccine and therapeutic protein candidates, including monoclonal antibodies, recombinant proteins, and virus-like particles.\n\nIndependently execute each step of the cell line development process: transfection, pool generation, single-cell cloning, clone expansion in static culture plates and shake flasks, fed-batch analysis, and clone stability studies.\n\nParticipate in cell line development platform design, optimization, and cycle-time reduction studies, including methods for clone selection, expansion, ranking, and characterization.\n\nGenerate stable pools and perform transient protein production to supply reagent-grade material.\n\nMaintain, support, and track maintenance for core laboratory equipment, including the ViCell BLU cell counters used on all cell line development projects to count cells during passaging and fed-batch production.\n\nSupport the planning and execution of research experiments within the laboratory’s overall research program.\n\nPrepare plasmid DNA using standard molecular cloning techniques, including polymerase chain reaction (PCR), restriction digest, and bacterial culture.\n\nUse automated platforms for clone selection and screening.\n\nSupport laboratory staff on research projects and techniques.\n\nPerform small-scale process development studies using various culture vessels, including tissue culture plates, shake flasks, and small-scale bioreactors.\n\nMaintain inventory of all supplies for the cell counters and request supplies as needed.\n\nTrain team members on proper cell counter usage, reagent pack replacement and disposal, and data retrieval.\n\nRespond the same day to team member requests for troubleshooting support.\n\nPerform monthly quality control (QC) and weekly maintenance on the cell counters.\n\nArrange service visits as needed and work with the vendor to ensure consistent performance of the instruments (currently five in the labs).\n\nMaintain a system of routine data backup.\n\nAssist in maintaining adequate space and facilities for ongoing and projected research studies.\n\nWrite and review standard operating procedures (SOPs) for cell line development processes and instrumentation.\n\nEvaluate, analyze, and interpret research data, and communicate results and methods in reports, presentations, and manuscripts.\n\nIndependently complete experiments and analyze data.\n\nWrite and review technical reports documenting process development studies and stable cell line generation work.\n\nMaintain complete documentation records, including a detailed and up-to-date laboratory notebook, to support regulatory filings.\n\nContribute results and studies for publication in peer-reviewed journals.\n\nCoordinate with junior staff to modify research protocols.\n\nWork with team members to generate experimental plans for process development studies.\n\nPresent research at local and/or national scientific meetings and symposia, including in poster form.\n\nProvide interpretation of results, guidance, and scientific support to the management team.\n\nCoordinate cell line development projects with the project lead and Federal Task Leader.\n\nPlan experiments to fit within required timelines.\n\nCommunicate any issues or delays promptly and help identify solutions.\n\nMaintain the cell line development master sheet with all critical information and data.\n\nRegularly communicate progress to the project lead and present work to the cell line development team.\n\nParticipate in project meetings.\n\nMentor junior-level scientists.\n\nTrain staff on proper equipment use and a wide variety of laboratory techniques.\n\nProvide technical experience to assist in studying the basic principles of plant and animal life.\n\nAssist with performing basic laboratory experiments and procedures.\n\nPrepare, organize, and maintain laboratory samples, supplies, and equipment.\n\nLearn new laboratory techniques and procedures.\n\nDisclaimer: The above description is meant to illustrate the general nature of work and level of effort being performed by individuals assigned to this position or job description. This is not restricted as a complete list of all skills, responsibilities, duties, and/or assignments required. Individuals may be required to perform duties outside of their position, job description or responsibilities as needed.\nThe diversity of Axle’s employees is a tremendous asset. We are firmly committed to providing equal opportunity in all aspects of employment and will not tolerate any illegal discrimination or harassment based on age, race, gender, religion, national origin, disability, marital status, covered veteran status, sexual orientation, status with respect to public assistance, and other characteristics protected under state, federal, or local law and to deter those who aid, abet, or induce discrimination or coerce others to discriminate.\nAccessibility: If you need an accommodation as part of the employment process please contact: \nThis role has a market-competitive salary with an anticipated base compensation range listed below. Actual salaries will vary depending on a candidate’s experience, qualifications, skills, and location.\nSalary Range\n$100,000—$110,000 USD","description_format":"text","description_chars":7714,"description_truncated":false,"requirements":{"experience_years_min":null,"management_years_min":null,"team_size_min":null,"manages_managers":false,"education":{"level":"bachelor","optional":false},"security_clearance":false,"languages":[]},"benefits":["401k plan"],"hiring_locations":[],"hiring_excludes":[],"relocation_offered":false,"industries":[],"lifecycle":[{"event":"open","at":"2026-10-15T16:58:48Z"}],"visa":[],"liveness":{"score":86,"band":"hot","label":"Hiring now","p_open":1,"p_active":0.86,"p_room":1,"age_days":1,"expected_fill_days":21,"reasons":["conf:2","win:early"],"computed_at":"2026-10-16T05:45:00Z"},"pay":{"stated_usd_annual":110000,"is_top_pay":false},"html_url":"https://alion.io/job/axleinfo-cell-line-development-scientist","json_url":"https://alion.io/job/axleinfo-cell-line-development-scientist.json","meta":{"generated_at":"2026-10-16T21:51:58Z","cache_seconds":300,"methodology":"https://alion.io/methodology","terms":"https://alion.io/terms","contact":"https://alion.io/contact","api":"https://alion.io/developers","about":"Alion is a live layer of people, companies and AI agents: who they are, whether they are real and active right now, what they do and how to work with them, readable by people and by agents and paid per call.","catalog":"https://alion.io/catalog.json","usage":{"tier":"crawler_verified","counted_by":"address","units_charged":1,"used_today":3097,"day_limit":null,"remaining_today":null,"minute_limit":300,"resets_at":"2026-10-17T00:00:00Z"}},"offers":[{"id":"company.slices","title":"One company in depth, by slice","status":"live","price":{"credits":0.02,"usd":0.002,"plus_per_slice":{"credits":0.05,"usd":0.005}},"unit":"per company, plus each slice with data","note":"the employer in depth","call":{"mcp_tool":"get_company","arguments":{"id":4416199},"rest":"https://alion.io/mcp/rest/get_company?id=4416199"},"human":"https://alion.io/catalog?offer=company.slices&for=job%2Faxleinfo-cell-line-development-scientist"},{"id":"market.stats","title":"A market slice: pay, demand and time to fill","status":"live","price":{"credits":1,"usd":0.1},"unit":"per slice","note":"pay, demand and time to fill for this role and place","call":{"mcp_tool":"market_stats"},"human":"https://alion.io/catalog?offer=market.stats&for=job%2Faxleinfo-cell-line-development-scientist"},{"id":"job.search","title":"Open jobs by role, technology, place, pay and visa","status":"live","price":{"credits":0.02,"usd":0.002},"unit":"per posting in a list","note":"similar open postings","call":{"mcp_tool":"search_jobs"},"human":"https://alion.io/catalog?offer=job.search&for=job%2Faxleinfo-cell-line-development-scientist"},{"id":"company.verify","title":"Is this company real and active right now","status":"pilot","price":null,"unit":"per company","request":{"url":"https://alion.io/catalog/request","method":"POST","body":"{\"offer\": \"company.verify\", \"for\": \"job/axleinfo-cell-line-development-scientist\", \"note\": \"what you need it for\"}"},"human":"https://alion.io/catalog?offer=company.verify&for=job%2Faxleinfo-cell-line-development-scientist"}]}