{"id":1975314,"url":"https://alion.io/job/ohalo-gene-editing-lab-technician-1st-shift","title":"Gene Editing Lab Technician (1st Shift)","company":{"id":3782230,"name":"Ohalo","domain":"ohalo.com","url":"https://alion.io/company/ohalo-2","size_band":"51-200","is_staffing_agency":false,"employer_type":"direct","is_intermediary":false,"listed_via":null,"ats_vendor":"Greenhouse","truth_index":{"grade":"A","score":91,"open_postings":6,"ghost_share":0,"stale_share":0.167,"repost_share":0,"time_to_fill_p50_days":85,"computed_at":"2026-11-01T06:10:25Z"}},"role":"Field & Service","role_family":"Field & Service","seniority":null,"employment_type":"full_time","work_mode":"on_site","remote_scope":null,"remote_scope_basis":null,"remote_working_hours":null,"hiring_geo_confidence":"structured","locations":["South San Francisco, United States"],"countries":["US"],"hiring_countries":[],"hiring_countries_total":0,"salary":{"min":29,"max":null,"currency":"USD","period":"hour","gross":null,"usd_annual":58000},"salary_estimate":null,"experience_years_min":null,"visa_sponsorship":true,"relocation_package":false,"has_equity":false,"technologies":[{"name":"Confocal Microscopy","optional":true},{"name":"GraphPad Prism","optional":true}],"status":"live","first_seen_at":"2026-10-06T19:29:08Z","employer_posted_date":"2026-10-30","last_verified_at":"2026-11-01T22:31:00Z","board_verified":true,"closed_at":null,"days_open":26,"trust":{"level":"ok","repost_count":1,"flags":[],"days_open":25},"description":"Position: Gene Editing Lab Technician\nShift: 1st Shift (7am - 3pm)\nLocation: SSF\nType: Full-time\nThe Opportunity: \nOhalo Genetics is seeking a motivated Gene Editing Lab Technician who will contribute to the advancement of plant variety development. The successful candidate will be detail-oriented, enthusiastic about hands-on work, and responsible. Prior experience in a sterile work environment, plant breeding or gene editing is a plus, but is not required. The Gene Editing Lab Technician will assist in specific duties to ensure the overall success of the gene editing laboratory. This role will involve additional responsibilities related to lab operations, plant inventory maintenance, and process development. This role is not limited to only lab work, and offers opportunities to contribute to various aspects of our lab's growth.\n\nResponsibilities: \n Protoplast Isolation, Transfection, and Culture:\nPerform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts.\nTransfect protoplasts using PEG-mediated transfection or electroporation methods.\nCulture transfected protoplasts to regenerate callus\nContribute to the continuous improvement and documentation of SOPs for protoplast workflows.\n Microscopy and Imaging:\nAssess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining).\n Data Recording and Analysis:\nMaintain accurate and detailed electronic lab notebooks (ELN) and batch records.\nCompile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel.\nSample Preparation:\nPrepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation.\nCross-Functional Support and Collaboration:\nCollaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed.\nLab Maintenance and Compliance:\nFollow safety protocols and institutional guidelines for working with plant tissue and enzymes.\nPerform all tasks in a manner consistent with and in support of all company policies and practices related to safety and compliance.\nPerforms other duties as assigned\nCandidate Profile: \nAbility to accurately follow verbal and written instructions as well as make suggestions to optimize methodologies \nAbility to sit for extended periods of time and do repetitive motions\nAbility to take a proactive approach to routine tasks\nMust be passionate about learning and enthusiastic about plants and hands-on work \nMust have a positive attitude and good interpersonal skills\nAbility to ask clarifying questions\nAbility to work independently and in a team environment with multiple departments\nMust be able to lift up to 50 pounds on intermittent basis and possess the ability to sit for extended periods of time\nCandidate must prioritize safety\nDesired skills/experience: \nExperience working in a lab including sterile technique, micropropagation, media preparation, microscopy, and DNA extraction\n\nBelow is the pay range for this position that we reasonably expect to pay. Individual compensation is based on various factors including experience, education, skillset, and other relevant qualifications. This range is for the South San Francisco, California location and may be adjusted to the labor market in other geographic areas.\nAnticipated Compensation: $29.50/hr\nAbout Ohalo: \nOhalo™ aims to accelerate evolution to unlock nature's potential. Founded in 2019, Ohalo develops novel breeding systems and improved plant varieties that help farmers grow more food with fewer natural resources, increasing the yield, resiliency, and genetic diversity of crops to sustainably feed our population. Ohalo's breakthrough technology, Boosted Breeding™, will usher in a new era of improved productivity to radically transform global agriculture. For more information, visit www.ohalo.com.\nNotes: If you previously applied for a job at Ohalo Genetics, we encourage you to restate your interest in the position by submitting your application.\nNo visa sponsorship is available for this position at this time. \nThis organization participates in E-Verify. Posters linked here. \nNo recruiters, please.","description_format":"text","description_chars":4245,"description_truncated":false,"requirements":{"experience_years_min":null,"management_years_min":null,"team_size_min":null,"manages_managers":false,"education":null,"security_clearance":false,"languages":[]},"benefits":[],"hiring_locations":[],"hiring_excludes":[],"relocation_offered":false,"industries":["Cell & Gene Therapy","Seeds"],"lifecycle":[{"event":"open","at":"2026-10-06T21:05:02Z"},{"event":"close","at":"2026-10-23T18:54:57Z"},{"event":"reopen","at":"2026-10-30T16:26:18Z"}],"visa":[{"country":"US","licensed_sponsor":true,"evidence":"H-1B filings in 12 months: 3","filings_12m":3,"filings_prev_12m":0,"green_card_filings_12m":0,"median_offered_wage_usd":132500,"route":null,"cap_exempt":false,"checked_at":"2026-10-29T16:27:21+00:00","sources":["US Department of Labor: LCA disclosure data (H-1B, H-1B1, E-3)"],"filings_for_role_12m":0}],"liveness":{"score":86,"band":"hot","label":"Hiring now","p_open":1,"p_active":0.858,"p_room":1,"age_days":25,"expected_fill_days":85,"reasons":["conf:3","win:early"],"computed_at":"2026-11-01T06:10:25Z"},"pay":{"stated_usd_annual":58000,"is_top_pay":false},"html_url":"https://alion.io/job/ohalo-gene-editing-lab-technician-1st-shift","json_url":"https://alion.io/job/ohalo-gene-editing-lab-technician-1st-shift.json","meta":{"generated_at":"2026-11-02T01:30:08Z","cache_seconds":300,"methodology":"https://alion.io/methodology","terms":"https://alion.io/terms","contact":"https://alion.io/contact","api":"https://alion.io/developers","about":"Alion is a live layer of people, companies and AI agents: who they are, whether they are real and active right now, what they do and how to work with them, readable by people and by agents and paid per call.","catalog":"https://alion.io/catalog.json","usage":{"tier":"crawler_verified","counted_by":"address","units_charged":1,"used_today":2314,"day_limit":null,"remaining_today":null,"minute_limit":300,"resets_at":"2026-11-03T00:00:00Z"}},"offers":[{"id":"company.slices","title":"One company in depth, by slice","status":"live","price":{"credits":0.02,"usd":0.002,"plus_per_slice":{"credits":0.05,"usd":0.005}},"unit":"per company, plus each slice with data","note":"the employer in depth","call":{"mcp_tool":"get_company","arguments":{"id":3782230},"rest":"https://alion.io/mcp/rest/get_company?id=3782230"},"human":"https://alion.io/catalog?offer=company.slices&for=job%2Fohalo-gene-editing-lab-technician-1st-shift"},{"id":"market.stats","title":"A market slice: pay, demand and time to fill","status":"live","price":{"credits":1,"usd":0.1},"unit":"per slice","note":"pay, demand and time to fill for this role and place","call":{"mcp_tool":"market_stats"},"human":"https://alion.io/catalog?offer=market.stats&for=job%2Fohalo-gene-editing-lab-technician-1st-shift"},{"id":"job.search","title":"Open jobs by role, technology, place, pay and visa","status":"live","price":{"credits":0.02,"usd":0.002},"unit":"per posting in a list","note":"similar open postings","call":{"mcp_tool":"search_jobs"},"human":"https://alion.io/catalog?offer=job.search&for=job%2Fohalo-gene-editing-lab-technician-1st-shift"},{"id":"company.verify","title":"Is this company real and active right now","status":"pilot","price":null,"unit":"per company","request":{"url":"https://alion.io/catalog/request","method":"POST","body":"{\"offer\": \"company.verify\", \"for\": \"job/ohalo-gene-editing-lab-technician-1st-shift\", \"note\": \"what you need it for\"}"},"human":"https://alion.io/catalog?offer=company.verify&for=job%2Fohalo-gene-editing-lab-technician-1st-shift"}],"agent":{"mcp":"https://alion.io/mcp/jobs","mcp_open":"https://alion.io/mcp/open","next":[{"tool":"get_job","arguments":{"id":1975314}},{"tool":"search_jobs","arguments":{"company":"ohalo.com","detail":"compact"}},{"tool":"match_people","arguments":{"job_id":1975314},"note":"people open to offers who fit this vacancy"}],"note":"The same data through MCP tools: https://alion.io/mcp/open needs no sign-in (compact searches, postings, people); https://alion.io/mcp with an Alion account has everything. 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