{"id":3923592,"url":"https://alion.io/job/pacbio-senior-scientist-biochemistry","title":"Senior Scientist, Biochemistry","company":{"id":58729,"name":"PacBio","domain":"pacb.com","url":"https://alion.io/company/pacbio","size_band":"201-500","is_staffing_agency":false,"employer_type":"direct","is_intermediary":false,"listed_via":null,"ats_vendor":"Workday","truth_index":null},"role":"Biotech","role_family":"Biotech","seniority":"senior","employment_type":"full_time","work_mode":"hybrid","remote_scope":null,"remote_scope_basis":null,"remote_working_hours":null,"hiring_geo_confidence":"explicit","locations":["Menlo Park, United States"],"countries":["US"],"hiring_countries":[],"hiring_countries_total":0,"salary":{"min":128800,"max":193200,"currency":"USD","period":"year","gross":null,"usd_annual":193200},"salary_estimate":null,"experience_years_min":2,"visa_sponsorship":true,"relocation_package":false,"has_equity":false,"technologies":[{"name":"Chromatography","optional":false},{"name":"Mass Spectrometry","optional":true},{"name":"Molecular Cloning","optional":true},{"name":"Next-Generation Sequencing","optional":true},{"name":"PyMOL","optional":true},{"name":"Python","optional":true}],"status":"live","first_seen_at":"2026-11-06T00:00:00Z","employer_posted_date":"2026-11-06","last_verified_at":"2026-11-13T05:51:41Z","board_verified":true,"closed_at":null,"days_open":7,"trust":{"level":"ok","repost_count":null,"flags":[],"days_open":7},"description":"Senior Scientist, Biochemistry R&D\nPacBio (NASDAQ: PACB) is a premier life science technology company that designs, develops, and manufactures advanced sequencing solutions to help scientists and clinical researchers resolve genetically complex problems. Our mission is to enable the promise of genomics to better human health. Genomics is core to all biological processes, and our advanced genomics tools provide scientists and clinical researchers the insights to better understand biology and health. We are now entering the century of biology and genomics is at the heart of the next revolution. Become part of the new paradigm in gene sequencing and help shape the future of genomic study by joining the PacBio team.\nAbout Biochemistry R&D:\nWe are PacBio's upstream research engine for third-generation sequencing chemistry and applications. Our team explores new enzymes, reaction chemistries, and molecular workflows, and tests feasibility of ideas that become PacBio's next products. When a concept works, it moves from our bench into Product Development and eventually onto sequencers in labs around the world. Some of our recent work spans HiFi library preparation, targeted enrichment of complex loci, and native detection of DNA modifications. We are a small, rigorous, and curious group. We pair hands-on biochemistry with sequencing data, work closely with Bioinformatics, Engineering, and Product teams, and give scientists real ownership of problems that matter.\nPosition Summary:\nPacBio is seeking a Senior Scientist to serve as the protein purification subject-matter expert within the Biochemistry and Reagents R&D group. This role combines creative problem-solving on challenging protein biochemistry with the operational rigor to execute at scale. The Senior Scientist will develop expression, purification, and characterization strategies for difficult and novel nucleic acid-modifying enzymes and their homologs; design quantitative biochemical assays that reveal how these enzymes work; and build and operate research-scale pipelines ranging from high-throughput 24- to 96-plex automated preparations to targeted individual purifications. The resulting well-characterized enzymes feed directly into PacBio's library preparation and sequencing chemistry research. The ideal candidate brings deep hands-on expertise in protein purification and mechanistic enzymology, the scientific creativity to solve problems where standard protocols fall short, and a collaborative approach to advancing the work of a multidisciplinary team. Experience applying these skills in an industry R&D environment is strongly preferred.\nResponsibilities:\nBuild, run, and continuously improve purification pipelines for nucleic acid-modifying enzymes (e.g., polymerases, ligases, nucleases, Cas proteins) at multiple research scales.\nServe as the group's subject-matter expert on protein expression and purification; advise colleagues on construct design, expression host, affinity tags, and purification strategy.\nDevelop expression and purification strategies for challenging targets, including poorly soluble proteins, multi-subunit complexes, and novel homologs, where standard protocols fall short; evaluate alternative strategies side by side to maximize yield, activity, and purity.\nDesign and execute high-throughput 24- to 96-plex expression and purification campaigns on lab automation platforms to screen enzyme variants and homologs in parallel.\nPerform targeted individual purifications on ÄKTA FPLC systems using affinity, ion-exchange, size-exclusion, and other chromatography methods.\nDesign and implement quantitative biochemical assays for new enzyme classes (e.g., steady-state and pre-steady-state kinetics, fidelity, and binding), including custom or modified nucleic acid substrates and plate-based activity screens integrated with high-throughput purification.\nEstablish and run QC for purified proteins (purity, concentration, activity, and contaminating nuclease testing) so material is fit for use in library preparation and sequencing chemistry experiments.\nPartner with scientists across Biochemistry and Reagents R&D to take new enzymes and homologs from expression through purification and QC to evaluation in PacBio library preparation and sequencing workflows.\nMaintain clear protocols, records, and a tracked inventory of purified enzyme lots.\nMentor and train Scientists and Research Associates in purification and QC methods.\nCommunicate results clearly in team meetings and written reports and contribute to cross-functional discussions.\nRequired Qualifications:\nPhD in Biochemistry, Molecular Biology, Biophysics, Chemistry, Bioengineering, or a related field, with a minimum of 2-5 years of relevant post-PhD research experience. Industry experience is strongly preferred.\nDeep hands-on expertise expressing and purifying recombinant proteins and protein complexes, ideally nucleic acid-binding or nucleic acid-modifying enzymes, including troubleshooting difficult targets.\nExpert user of FPLC systems with strong command of affinity, ion-exchange, and size-exclusion chromatography and method development.\nStrong foundation in mechanistic enzymology and quantitative biochemical assay development (e.g., enzyme kinetics, fluorescent or radiolabeled substrate assays, binding measurements).\nDemonstrated experience running high-throughput (24- to 96-plex) protein purification on lab automation or liquid-handling platforms (e.g., KingFisher, Tecan, Opentrons, Hamilton).\nProficiency in protein QC and characterization (e.g., SDS-PAGE, capillary electrophoresis, A280 quantitation, DLS, activity and nuclease contamination assays).\nTrack record of delivering high-quality reagents while balancing multiple concurrent projects and requests.\nConsummate team player who enjoys enabling the success of colleagues across a multidisciplinary group.\nExcellent interpersonal, written, and verbal communication skills.\nPreferred Experience:\nIndustry R&D experience in biotechnology, life science tools, or enzyme and reagent development, including supporting product development programs or working alongside Manufacturing and Quality teams.\nFamiliarity with single-molecule biochemistry or biophysics.\nFamiliarity with DNA sequencing, especially NGS library preparation or long-read sequencing (PacBio HiFi or Oxford Nanopore).\nExperience with biochemical assays for polymerases, ligases, nucleases, and/or Cas proteins, including enzyme kinetics.\nExperience with construct design, molecular cloning, and expression optimization (e.g., codon optimization, solubility tags, host selection).\nExperience with biophysical characterization methods (e.g., DLS, intact mass spectrometry, thermal shift).\nStructural biology experience (e.g., X-ray crystallography or cryo-EM), particularly with protein-nucleic acid complexes.\nProtein engineering experience, such as site-directed or site-saturation mutagenesis libraries or directed evolution.\nSoftware Experience: data analysis in Python or R, custom database management and structure visualization (e.g., Chimera, PyMOL, Coot).\nCandidates must have current authorization to work in the United States without the need for present or future sponsorship.\nNon-Field Based Employees are required to be onsite Monday-Thursday (Friday work from home). Depending on the role, some employees may be required to be 100% onsite.\nYou may be required from time to time to visit and work at PacBio locations and for such times as the Company considers necessary for the proper performance of your duties.\nAll listed tasks and responsibilities are deemed as essential functions to this position; however, business conditions may require reasonable accommodations for additional tasks and responsibilities.\nAll qualified applicants will receive consideration for employment without regard to race, sex, color, religion, national origin, protected veteran status, or on the basis of disability, gender identity, and sexual orientation.\nSalary Range:\n$128,800.00 - $193,200.00To all Staffing and Recruiting Agencies: Our Careers Site is only for individuals seeking a job at PacBio. Staffing and recruiting agencies and individuals being represented by an agency are not authorized to use this site or to submit profiles, applications or resumes, and any such submissions will be considered unsolicited. PacBio does not accept unsolicited resumes or applications from agencies. Please do not forward resumes to our jobs alias, PacBio employees or any other company location. PacBio is not responsible for any fees related to unsolicited resumes/applications.\nAll qualified applicants will receive consideration for employment without regard to race, sex, color, religion, national origin, protected veteran status, or on the basis of disability, gender identity, and sexual orientation.\nIf you are an individual with a disability and require a reasonable accommodation to complete any part of the application process, or are limited in the ability or unable to access or use this online application process and need an alternative method for applying, you may contact us at 650-521-8000, or  for assistance.\nVisit our following pages for more information on:\nFAQs\nBenefits\nCulture\nEqual Opportunity Employment\nBeware of Recruitment Fraud\nPlease be aware of employment scams where people or entities misuse PacBio’s name, or the names of PacBio employees, to post fake job advertisements or make fake job offers.\nPacBio will never extend a job offer without a personal interview and will never charge any money for joining our company. All current job openings are displayed on the Careers page of our official website.\nIf you see a job posting that is not on the PacBio official website, or have been approached with a job offer that you suspect may be fraudulent, we strongly recommend you do not respond and do not send money or provide any personal information.\nIT IS LIKELY A SCAM OR FAKE JOB AD IF:\nThe job offer requires youto pay something (for example, to purchase equipment).\nYou receive a job offer that you didn’t apply for, or without a personal interview or interaction.\nThe interview seems to involve AI video or audio.\nYou found a job on a job board or third-party channel, but the job doesn’t exist on the official PacBio careers page\nYou suspect it is a scam.\nHOW TO PROTECT YOURSELF:\nNever give personal and confidential information.\nNever pay for anything as part of interviewing for, or accepting, an offer of employment.\nIgnore it if it seems like a scam.","description_format":"text","description_chars":10564,"description_truncated":false,"requirements":{"experience_years_min":2,"management_years_min":null,"team_size_min":null,"manages_managers":false,"education":{"level":"phd","optional":false},"security_clearance":false,"languages":[]},"benefits":[],"hiring_locations":[{"name":"United States","iso":"US","kind":"country"}],"hiring_excludes":[],"relocation_offered":false,"industries":["Biotechnology","Health Care","Genomics & Genetic Testing","Laboratory Equipment"],"lifecycle":[{"event":"open","at":"2026-11-07T17:11:21Z"}],"visa":[],"liveness":{"score":86,"band":"hot","label":"Hiring now","p_open":1,"p_active":0.86,"p_room":1,"age_days":6,"expected_fill_days":50,"reasons":["conf:4","win:early"],"computed_at":"2026-11-12T10:38:37Z"},"pay":{"stated_usd_annual":193200,"is_top_pay":true},"html_url":"https://alion.io/job/pacbio-senior-scientist-biochemistry","json_url":"https://alion.io/job/pacbio-senior-scientist-biochemistry.json","meta":{"generated_at":"2026-11-13T16:11:21Z","cache_seconds":300,"methodology":"https://alion.io/methodology","terms":"https://alion.io/terms","contact":"https://alion.io/contact","api":"https://alion.io/developers","about":"Alion is a live layer of people, companies and AI agents: who they are, whether they are real and active right now, what they do and how to work with them, readable by people and by agents and paid per call.","catalog":"https://alion.io/catalog.json","usage":{"tier":"crawler_verified","counted_by":"address","units_charged":1,"used_today":32458,"day_limit":null,"remaining_today":null,"minute_limit":300,"resets_at":"2026-11-14T00:00:00Z"}},"offers":[{"id":"company.slices","title":"One company in depth, by slice","status":"live","price":{"credits":0.1,"usd":0.01,"plus_per_slice":{"credits":0.05,"usd":0.005}},"unit":"per company, plus each slice with data","note":"the employer in depth","call":{"mcp_tool":"get_company","arguments":{"id":58729},"rest":"https://alion.io/mcp/rest/get_company?id=58729"},"human":"https://alion.io/catalog?offer=company.slices&for=job%2Fpacbio-senior-scientist-biochemistry"},{"id":"market.stats","title":"A market slice: pay, demand and time to fill","status":"free","price":{"credits":0,"usd":0},"unit":"per slice","note":"pay, demand and time to fill for this role and place","call":{"mcp_tool":"market_stats"},"human":"https://alion.io/catalog?offer=market.stats&for=job%2Fpacbio-senior-scientist-biochemistry"},{"id":"job.search","title":"Open jobs by role, technology, place, pay and visa","status":"live","price":{"credits":0.1,"usd":0.01},"unit":"per posting in a list","note":"similar open postings","call":{"mcp_tool":"search_jobs"},"human":"https://alion.io/catalog?offer=job.search&for=job%2Fpacbio-senior-scientist-biochemistry"},{"id":"company.verify","title":"Is this company real and active right now","status":"pilot","price":null,"unit":"per company","request":{"url":"https://alion.io/catalog/request","method":"POST","body":"{\"offer\": \"company.verify\", \"for\": \"job/pacbio-senior-scientist-biochemistry\", \"note\": \"what you need it for\"}"},"human":"https://alion.io/catalog?offer=company.verify&for=job%2Fpacbio-senior-scientist-biochemistry"}],"agent":{"mcp":"https://alion.io/mcp/jobs","mcp_open":"https://alion.io/mcp/open","next":[{"tool":"get_job","arguments":{"id":3923592}},{"tool":"search_jobs","arguments":{"company":"pacb.com","detail":"compact"}},{"tool":"match_people","arguments":{"job_id":3923592},"note":"people open to offers who fit this vacancy"}],"note":"The same data through MCP tools: https://alion.io/mcp/open needs no sign-in (compact searches, postings, people); https://alion.io/mcp with an Alion account has everything. Docs: https://alion.io/developers"}}