Lab Technician I - Laboratory

Laboratory Technician

Job Summary:  Medical Diagnostic Laboratories(MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses.

MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).

MDL is looking for a Laboratory Technician. The Laboratory Technicians responsible for printing labels and attaching requisitions to the corresponding specimens.

Essential Functions: 

Accessioning:

Print labels and attach to requisitions and the corresponding specimens

Properly accession multiple specimen types

Take photographs of all specimens received

Properly identify any discrepant information

File all paperwork in order and distribute to the appropriate QC bins

Properly scan accessioned requisitions

Scan 2-D barcode elution tubes used for extraction

Properly locate and record temperatures for all necessary stations

Manual Extraction:

Perform Phenol/Chloroform manual extractions on biopsies and ticks

Properly separate samples into their organic and inorganic layers via phenol/chloroform

Properly perform quantitative analysis and hydrate all ticks and biopsies

Robotic Extraction:

Properly extract all samples types using a provided XG sheet as a template

Properly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagents

Properly dispose of all waste material when required; both hazardous and non-hazardous

Attend to robotics to address any user interactions and/or robotic malfunctions

Manually extract all necessary samples via commercial kits

RNA Extraction:

Perform manual RNA extractions via Qiagen kit for all necessary samples

Perform robotic RNA extractions via VX Reagents kit for all necessary samples

PCR:

Perform manual and robotic Conventional method PCR amplification

Properly load and start thermocycler machines

Perform manual and robotic Real-time method PCR amplification

Properly load and start Rotor-gene thermocycler

Perform initial analysis on completed Real-time PCR

Perform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic Dispensor

Properly load and start Bio-Rad CFX thermocycler

Perform initial analysis on completed Acoustic Real-Time PCR

 Gel Electrophoresis:

Properly identify which completed PCRs from the thermocycler require gel electrophoresis

Properly identify when to make and/or use 2% versus 2.5% gels

Complete blank gel template sheets to combine short PCR sheets

Use dye to stain all necessary samples with the appropriate volume on a test by test basis

Properly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)

Properly capture a visual image of the gel with the use of a SYNGENE UV gel reader

Be able to troubleshoot any gel box or power source errors, as well as any gel reader errors

Pyrosequencing

Properly perform nested PCR prior to sequencing

Properly load and run Sequencing Analysis reactions (SQA)

Properly load and run Single Nucleotide Polymorphism reactions (SNP)

Properly analyze all run data

Micro-Biology/Cell Culture

Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single colonies

Properly determine specific species present, and inoculate appropriate plate with said species on a quad plate preloaded with antibiotics, or an agar plate stamped with antibiotic discs

Properly analyze growth plate to determine antibiotic susceptibility or resistance

For GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic Chamber System

Identify and pick colonies for PCR

Perform CAMP test and analyze for zones of hemolysis

Next-Generation Sequencing

Properly perform a series of nested PCRs for BRCA, HPV, and Cystic Fibrosis

Properly attach samples to appropriate bead mixtures

Properly perform beads cleaning to remove unused dNTP’s and primers within the amplified PCR product

Properly perform gel purification with the use of the Pippin Prep machine

Properly load and operate multiple Life Technologies Equipment

Properly analyze all run data

 Additional Responsibilities:

Performs other similar or related duties as required or assigned.

Assures activities are conducted in a safe manner and materials are handled according to safety guidelines.

Assures customer privacy is maintained at all times.

Participates as a team member and is accountable for own work responsibilities.

Assures information is delivered in a manner that is supportive, timely and understandable.

Initiative is demonstrated to proactively diagnose and resolve problems. 

Productive work habits are consistently displayed.

Accuracy and quality are maintained for all activities. 

Job Qualifications:  General Knowledge, Skills, and Abilities (KSA’s) required 

9-month Clinical Laboratory Technician training

Physical Demands: Physical, Mental and Workplace Environment Conditions

Ability to sit, stand, walk and perform repetitive motion (manual pipette)

Proficient visual and audio skills

Workplace Conditions: Workplace Environment Conditions

Laboratory setting

Use of personal protective equipment may be necessary

May be exposed to chemicals, blood/body fluids

Education and Certifications: 

Bachelor Degree in Biology, Chemistry, or related science field

We Offer:

•             Competitive Salary

•             Medical, Dental & Vision Insurance

•             Short and Long Term Disability

•             Life Insurance

•             Dependent Care Flex Spending Account

•             Voluntary Policies (Accident, Hospital Indemnity, Critical Illness & Supplemental STD)

•            Identity Theft Protection

•             401(k) Plan

•             Paid Vacation & Holiday

•             Business Casual Environment

Visit us at https://www.genesisglobalgrp.com/

Genesis Biotechnology Group is a member of Genesis Global Group, an equal opportunity employer.