Research Associate II / SR.

Research Associate II/Sr.

We are seeking a highly driven and meticulous researcher to join a team developing the next generation of CRISPR-based therapeutics. The current role is for a Research Associate skilled in working with mammalian tissue culture (specifically neural cell types), AAV vectors and molecular biology. The role will work collaboratively across interdisciplinary teams, including protein engineers, process sciences, computational biologists, NGS, and disease experts.

Key Responsibilities:

• Plan and execute in vitro cellular and molecular biology assays, analyze, and document results to support both our molecule engineering platform and CNS biology discovery workstreams.
• Provide robust, hands-on mammalian tissue culture (TC) support, including routine maintenance and handling of engineered and primary/complex cell lines.
• Optimize and validate molecular and cell-based assays to support screening.
• Perform and own routine molecular biology workflows, such as plasmid cloning, bacterial transformations, and minipreps/maxipreps.
• Run quantitative molecular assays (e.g. CellTiter glow, ELISA, qPCR, ddPCR…) to analyze target efficacy and gene editing outcomes.
• Communicate research plans and results to team members verbally and in writing.
• Effectively collaborate with members within a fully integrated team to facilitate execution on projects within established timelines.

Required Skills and Background:

• A Bachelor’s and/or Masters in Biology, Neuroscience, Cell biology, Molecular Biology, or related disciplinewith 3+ years (Msc) or 5+ years (Bsc) relevant hands-on experience with methods analyzing DNA, RNA,and protein (ELISA, qPCR, ddPCR, flow cytometry, western blot, and other relevant techniques).
• Cell culture experience with relevant primary and immortalized cell lines (iPSC-derived neurons, primary neurons, HEK, SH-SY5Y) is essential.
• Proficiency in molecular biology techniques common in upstream research, including molecular cloningand processing genomic DNA and RNA for downstream analysis (qPCR, ddPCR, NGS) is required.
• Ability to work both independently and collaboratively in a fast-paced, interdisciplinary research team.

Preferred Skills and Background:

• Experience with different cellular transfection and transduction methods (AAV, LV, lipofection).
• Experience with CRISPR-Cas gene editing systems and delivery technology.
• Experience with high-throughput or high-content assays.
• Working knowledge of molecular biology software.
• Previous Pharma, Biotech or CRO experience.