Zhixiang Liu

Performed hands-on LC-MS/MS bioanalysis for large-molecule samples, including proteins, peptides, and antibody-drug conjugates. Operated Shimadzu Nexera X2 UHPLC for chromatographic separation and prepared biological samples for injection. Built and evaluated calibration curves, supported quantitative result processing, and participated in GLP method validation. Managed sample and data workflows using Watson LIMS with GLP data integrity documentation.

Conducted core flooding experiments and wettability/relative permeability measurements to assess reservoir behavior. Performed nitrogen/CO2 Huff-n-Puff optimization and used fractional-flow style analysis to evaluate surfactant-mediated wettability changes. Applied established correlations and analytical methods to interpret reservoir performance.

Developed membrane-coated and hybrid nanocarriers and assessed membrane protein retention, physicochemical properties, in vitro transfection, and in vivo biodistribution. Worked on lipid-based nanobubbles intended for mRNA and oxygen co-delivery. Evaluated stability and ultrasound-triggered release behavior in vitro and in vivo.

Engineered membrane-coated nanoparticle platforms using red blood cell, platelet, tumor cell, and bacterial membrane materials to improve circulation and immune evasion. Built hybrid carriers by combining membrane coatings with LNP or polymeric cores to tune biological interfaces. Evaluated membrane protein retention and performed cell transfection and in vivo biodistribution/efficacy studies.

Developed and optimized mRNA-loaded lipid nanoparticles (LNPs) and liposomes, combining formulation work with analytical testing. Applied chromatographic and particle characterization methods to assess formulation attributes such as residual solvent content and particle morphology. Designed and evaluated a continuous manufacturing approach using residual solvent analysis and experimental design to support critical quality attribute targets.